Lysates were pre-cleared with Sepharose 6B (Sigma-Aldrich), and immunoprecipitated overnight in 4C with G-protein Sepharose beads (Amersham Biosciences, Les Ullis, France)
Lysates were pre-cleared with Sepharose 6B (Sigma-Aldrich), and immunoprecipitated overnight in 4C with G-protein Sepharose beads (Amersham Biosciences, Les Ullis, France). due to its capability …